Cost-Effective DNA Test For Crime Scenes and Disease Diagnosis

DNA Test For Crime Scenes and Disease Diagnosis Could Be More Cost-Effective according to a new technique developed by Scientist in Japan which improved the already Known method of the fabled polymerase chain reaction (PCR).

PCR is a technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of a particular DNA sequence this allow Scientist and investigators to detect or find DNA in cases where there is a very strong chance of not finding any usable sample.

This new technique not only will reduce cost at the time of doing the test but also will open new opportunities in the field of medicine and criminal studies since it will lead to new methods in the usage of  PCR.

This new method consists in , a quenching probe (QProbe) and a nonfluorescent 3′-tailed probe are used. The QProbe is a singly labeled oligonucleotide bearing a fluorescent dye that is quenched via electron transfer between the dye and a guanine base at a particular position. The nonfluorescent 3′-tailed probe consists of two parts: one is the target-specific sequence on the 5′ side, and the other is complementary to the QProbe on the 3′ side. When the QProbe/nonfluorescent 3′-tailed probe complex hybridizes with the target in PCR, the fluorescence of the dye is quenched. Fluorescence quenching efficiency is proportional to the amount of the target

This method substantially reduces the cost of real-time PCR setup because the same QProbe can be used for different target sequences. Moreover, this method allows accurate quantification even in the presence of nonspecific PCR products because the use of nonfluorescent 3′-tailed probe significantly increases specificity.

~ by avissuetti on July 9, 2009.